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Hi Jenny, First, the quench in H2O2 is to quench or "use up" endogenous peroxidases so that the substrate is only catalyzed by the HRP, and not endogenous peroxidase. Case Western Reserve University receives $3.7M NIH grant to study autonomic nervous system link to painful bladder syndrome. CLEVELAND July 23, 2009 Case Western. In article <fergusb-0112951158130001@morrison3.mbi.ucla.edu>, fergusb@microbio.lifesci.ucla.edu says. > >WELL KLH IS THE USUSAL OTHER OPTION BUT I DO REMEMBER HEARING. Hi Jenny, First, the quench in H2O2 is to quench or "use up" endogenous peroxidases so that the substrate is only catalyzed by the HRP, and not endogenous peroxidase. Case Western Reserve University receives $3.7M NIH grant to study autonomic nervous system link to painful bladder syndrome. CLEVELAND July 23, 2009 Case Western. In article <fergusb-0112951158130001@morrison3.mbi.ucla.edu>, fergusb@microbio.lifesci.ucla.edu says. > >WELL KLH IS THE USUSAL OTHER OPTION BUT I DO REMEMBER HEARING.